Biomolecules Introduction, classification, chemical nature and biological role of carbohydrate, lipids, nucleic acids, amino acids and proteins.

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Bioenergetics Concept of free energy, endergonic and exergonic reaction, Relationship between free energy, enthalpy and entropy; Redox potential.

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Energy rich compounds; classification; biological significances of ATP and cyclic AMP

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Glycolysis – Pathway, energetics and significance

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Citric acid cycle- Pathway, energetics and significance

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HMP shunt and its significance; Glucose-6-Phosphate dehydrogenase (G6PD) deficiency

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Glycogen metabolism Pathways and glycogen storage diseases (GSD)

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Gluconeogenesis- Pathway and its significance

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Hormonal regulation of blood glucose level and Diabetes mellitus

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Electron transport chain (ETC) and its mechanism.

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Oxidative phosphorylation & its mechanism and substrate phosphorylation

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Inhibitors ETC and oxidative phosphorylation/Uncouplers

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Disorders of lipid metabolism

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General reactions of amino acid metabolism

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Catabolism of heme

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Genetic code, Translation or Protein synthesis and inhibitors

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Introduction, properties, nomenclature and IUB classification of enzymes

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β-Oxidation of saturated fatty acid (Palmitic acid)

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Formation and utilization of ketone bodies; ketoacidosis

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De novo synthesis of fatty acids (Palmitic acid)

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Catabolism of phenylalanine and tyrosine and their metabolic disorders

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Synthesis and significance of biological substances

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Catabolism of purine nucleotides and Hyperuricemia and Gout disease

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Organization of mammalian genome

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Structure of DNA and RNA and their functions

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DNA replication (semi conservative model)

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Transcription or RNA synthesis

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Enzyme kinetics (Michaelis plot, Line Weaver Burke plot)

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Enzyme inhibitors

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Regulation of enzymes: enzyme induction and repression, allosteric enzymes regulation

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